Guillotine is a level in which your monkey ball is supposed to roll up ramps that become longer and longer in a semi-circle. A spinning rod holds two platforms that wipe across the top of the ramps. Therefor, if you don't make it over the ramp near it's edge, there is a high chance that you will be knocked off the edge of the course with the one of two "guillotines."
If I'm not mistaken, the timing of this level is 60 seconds.
The difficulty of this level is advanced compared to an average player.